1.Umuco w'akagari ni iki?
Umuco w'akagari bivuga kuvana ingirabuzimafatizo ku nyamaswa cyangwa ibimera hanyuma ukabikurira ahantu heza.Ingirabuzimafatizo zishobora gukurwa mu buryo butaziguye hanyuma zigacika hakoreshejwe uburyo bwa enzymatique cyangwa ubukanishi mbere yo gutsimbataza, cyangwa zishobora gukomoka ku mirongo yashizweho cyangwa imirongo ya selile.
2.Umuco wibanze ni uwuhe?
Umuco wibanze bivuga icyiciro cyumuco nyuma yuko selile zitandukanijwe nuduce hanyuma zikagwira mugihe gikwiye kugeza zifata insimburangingo zose zishoboka (ni ukuvuga kugera ihuriro).Kuri iki cyiciro, ingirabuzimafatizo zigomba kuba zifite umuco wo kuzimurira mu kintu gishya gifite uburyo bushya bwo gukura kugira ngo zitange umwanya munini wo gukomeza gukura.
2.1 Umurongo w'akagari
Nyuma ya subculture yambere, umuco wibanze witwa umurongo wa selile cyangwa subclone.Imirongo ya selile ikomoka kumico yibanze ifite igihe gito cyo kubaho (nukuvuga ko ari nto; reba hepfo), kandi uko irengana, ingirabuzimafatizo zifite ubushobozi bwo gukura cyane ziriganje, bikavamo urwego runaka rwa genotype mubaturage batabana na phenotype.
2.2 Ingirabuzimafatizo
Niba subpopulation y'umurongo w'akagari yatoranijwe neza mumuco ukoresheje cloni cyangwa ubundi buryo, umurongo w'akagari wahinduka ingirabuzimafatizo.Uturemangingo ngengabuzima tubona impinduka zinyuranye nyuma yumurongo wababyeyi utangiye.
3.Imirongo ngari kandi ikomeza
Ingirabuzimafatizo zisanzwe zigabanya inshuro nke gusa mbere yo gutakaza ubushobozi bwo kwiyongera.Iki nikintu cyagenwe genetiki cyitwa senescence;iyi mirongo ya selile yitwa imirongo ya selile.Nyamara, imirongo imwe nimwe ya selile iba idapfa binyuze mubikorwa byitwa guhinduka, bishobora kubaho ubwabyo cyangwa bishobora guterwa nimiti cyangwa virusi.Iyo umurongo utagira ingano ugizwe nimpinduka kandi ukagira ubushobozi bwo kugabana ibihe bitarondoreka, bihinduka umurongo uhoraho.
4.Umuco
Imiterere yumuco ya buri bwoko butandukanye iratandukanye cyane, ariko ibidukikije byubukorikori bwo guhuza ingirabuzimafatizo bigizwe nibintu byabigenewe, birimo ibi bikurikira:
4.1 Substrate cyangwa umuco urwego rutanga intungamubiri zingenzi (acide amine, karubone, vitamine, imyunyu ngugu)
4.2 Impamvu zo gukura
4.3 Imisemburo
4.4 Imyuka (O2, CO2)
4.5 Ibidukikije byumubiri nubumara (pH, umuvuduko wa osmotic, ubushyuhe)
Ingirabuzimafatizo nyinshi zishingiye kuri anchorage kandi zigomba kuba zifite umuco kuri substrate ikomeye cyangwa igice gikomeye (umuco wa adherent cyangwa monolayer), mugihe izindi selile zishobora gukura zireremba hagati (umuco wo guhagarika).
5.Gukingira
Niba hari selile zirenze urugero muri subculture, zigomba kuvurwa hamwe nuburyo bukingira (nka DMSO cyangwa glycerol) hanyuma bikabikwa ku bushyuhe buri munsi ya -130 ° C (cryopreservation) kugeza bikenewe.Kubindi bisobanuro bijyanye na subculture na cryopreservation ya selile.
6.Morphologiya y'utugingo ngengabuzima
Ingirabuzimafatizo mu muco zishobora kugabanywamo ibyiciro bitatu by'ibanze ukurikije imiterere n'imiterere (ni ukuvuga morphologie).
6.1 Fibroblast selile ni bipolar cyangwa multipolar, ifite ishusho ndende, kandi ikura ifatanye na substrate.
6.2 Epiteliyale isa na selile ni polygonal, ifite ubunini busanzwe, kandi ifatanye na matrix mumpapuro zidasanzwe.
6.3 Lymphoblast imeze nkutugingo ngengabuzima kandi ubusanzwe ikura muguhagarikwa nta kwizirika hejuru.
7.Gukoresha umuco w'akagari
Umuco w'akagari ni kimwe mu bikoresho by'ingenzi bikoreshwa mu binyabuzima na selile.Itanga uburyo bwiza bwicyitegererezo cyo kwiga physiologie isanzwe na biohimiya yingirabuzimafatizo (nkubushakashatsi bwa metabolike, gusaza), ingaruka zibiyobyabwenge nuburozi bwubumara kuri selile, na mutagenezi ningaruka za kanseri.Ikoreshwa kandi mugupima ibiyobyabwenge no kwiteza imbere no gukora inganda nini zikora ibinyabuzima (nkinkingo, proteyine zo kuvura).Inyungu nyamukuru yo gukoresha umuco wutugari kuri buri kintu icyo aricyo cyose ni uguhuza no kubyara ibisubizo bishobora kuboneka ukoresheje icyiciro cya selile.
Igihe cyo kohereza: Jun-03-2019